Ginkgo Cloud Lab

Overview

Ginkgo Cloud Lab (https://cloud.ginkgo.bio) provides remote access to Ginkgo Bioworks' autonomous lab infrastructure. Protocols are executed on Reconfigurable Automation Carts (RACs) -- modular units with robotic arms, maglev sample transport, and industrial-grade software spanning 70+ instruments.

The platform also includes EstiMate, an AI agent that accepts human-language protocol descriptions and returns feasibility assessments and pricing for custom workflows beyond the listed protocols.

The catalog is organized into Expression & Purification (in vitro / cell-free / E. coli / Pichia), Characterization & Assay, Method & Target Onboarding, and Specialty. Pick a protocol below, then read its reference file for inputs, outputs, the automated workflow, and ordering details.

Available Protocols

Expression & Purification - In vitro

Protocol	Readout	Price	Turnaround	Status
IVT mRNA/circRNA Synthesis	qPCR (mRNA or circRNA, 384-well)	$99/sample	up to 12 business days	Certified

Expression & Purification - Cell-free (E. coli CFPS)

Protocol	Readout	Price	Turnaround	Status
Validate sequence expression	Go/no-go titer + purity (up to 1800 bp)	$39/sample	up to 10 days	Certified
Optimize expression conditions	DoE across 24 conditions	$199/sample	up to 11 days	Certified
Express + quantify (HiBiT)	Luminescence, no purification	$39/sample	up to 11 days	Certified
Express + purify (A280)	Strep-tag, A280 yield	$149/sample	up to 11 days	Certified
Express + purify minibinder	Strep-tag, A280, LabChip	$149/sample	up to 11 days	Certified
Express + purify (A280 + LabChip)	Strep-tag, A280 + purity/size	$159/sample	up to 12 days	Certified

Expression & Purification - E. coli

Protocol	Readout	Price	Turnaround	Status
Express + quantify (HiBiT)	Luminescence (up to 384 constructs)	$79/sample	up to 3 weeks	Certified
Express + purify (A280)	His-tag, A280 yield	$199/sample	up to 3 weeks	Certified
Express + purify minibinder	His-tag, A280 yield	$199/sample	up to 3 weeks	Certified
Express + purify (A280 + LabChip)	His-tag, A280 + purity/size	$209/sample	up to 3 weeks	Certified

Expression & Purification - Pichia

Protocol	Readout	Price	Turnaround	Status
Express + quantify (LabChip)	Secreted protein, size/purity (up to 96)	$89/sample	up to 4 weeks	Certified (New)

Characterization & Assay

Protocol	Readout	Price	Turnaround	Status
Express + thermal shift	SYPRO Orange Tm (Tonset, TM1-3)	$159/sample	up to 12 days	Certified
Detect enzymatic products (Echo-MS)	Substrate/product by Echo-MS	$44/sample	up to 13 days	Beta

Method & Target Onboarding

Protocol	Readout	Price	Turnaround	Status
Onboard Echo-MS method	Calibration curve, LOD/LOQ	$799/molecule	up to 3 weeks	Certified
Onboard SPR target	Validated SPR capture method	$1,399/target	up to 4 weeks	Beta

Specialty

Protocol	Readout	Price	Turnaround	Status
Generate fluorescent pixel art	UV photo, 7-color E. coli palette	$25/plate	up to 7 days	Beta

Coming soon: Protein Expression and Binding Affinity Characterization (express + purify, then screen binding affinity against a target).

Choosing a Protocol

• Quick expressibility screen? Cell-free HiBiT ($39) or Validate sequence expression ($39).
• Need purified protein + yield? A280 tiers (cell-free or E. coli); add LabChip for purity/size.
• Difficult / membrane / disulfide / cofactor targets? Cell-free Optimize (24-condition DoE).
• Secreted or eukaryotic targets? Pichia expression.
• Screening de novo binders/minibinders? Cell-free or E. coli minibinder tiers, then SPR onboarding for kinetics.
• Enzyme activity / biocatalysis? Echo-MS enzymatic detection (onboard the analyte method first).
• Stability / developability ranking? Thermal shift assay.
• RNA (mRNA/circRNA)? IVT synthesis + qPCR.

General Ordering Workflow

1. Select a protocol at https://cloud.ginkgo.bio/protocols
2. Configure parameters (number of proteins/samples/molecules/targets, replicates, plates)
3. Download the protocol's input template and upload inputs (FASTA/CSV/XLSX for sequence protocols; Design Tool for pixel art; vendor catalog numbers for onboarding)
4. Add any special requirements in the Additional Details field
5. Provide an email, agree to the protocol terms, and add to cart / submit to receive a feasibility report and price quote

For protocols not listed above, use the EstiMate chat (https://cloud.ginkgo.bio/estimate) to describe a custom protocol in plain language and receive a compatibility assessment and pricing.

Authentication

Access Ginkgo Cloud Lab at https://cloud.ginkgo.bio. Account creation or institutional access may be required. Contact Ginkgo at [email protected] for access questions.

Key Infrastructure

• RACs (Reconfigurable Automation Carts): Modular robotic units with high-precision arms and maglev transport
• Catalyst Software: Protocol orchestration, scheduling, parameterization, and real-time monitoring
• 70+ integrated instruments: Agilent Bravo liquid handlers, Beckman/Labcyte Echo acoustic dispensers, BMG PHERAstar / Tecan Spark readers, Revvity LabChip, Bio-Rad CFX Opus, Nicoya Alto SPR, SciEx Echo-MS, Inheco/Cytomat incubators, and more
• Nebula: Ginkgo's autonomous lab facility in Boston, MA